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This project involves processing tissue samples for RNA isolation, cDNA synthesis, and quantitative PCR (qPCR) to measure the expression of various cytokines—key cell signaling molecules. Successful candidates will also be responsible for culturing cells and maintaining sterile monocultures. Our research focuses on understanding how cancer cells respond to specific metabolites, including the neurotransmitter serotonin. Students will receive training in western blotting to assess protein expression using antibodies and horseradish peroxidase tags, enabling quantification of protein levels within a given sample. Each of these techniques is highly transferable and valuable for students pursuing careers in graduate or medical school after completing their degree at Berry College. |